English
Materiae et Methodi@___@3.1. Excutere Flask Cultus
Praeculturae MDCK.SUS2 Cellae suspensionis in
creverunt (125 mL ampullae polycarbonatae Erlenmeyer, #431143, Corning®, Urbs Novi Eboraci, NY, USA) cum 50 mL volumen operans (wr) , in a Multitron Pro incubator (Infors HT, Bottmingen, Helvetia) ad 37 °C et 5% CO2 atmosphaera cum frequentia 180 rpm quatiens. Cellae singulae 3-4 diebus perductae sunt cum seminis densitate 0.5-0.8 × 106 cellae/mL.IAV A/Puerto Rico/8/34 (H1N1) semen virus adhibitum ad infectionem generatam. in cellulae adhaerens MDCK (ECACC 84121903). Virus semen habebat titer 1.1 × 109 TCID50/mL. flasks
Cellae chemicae definitae colebantur, interdum libero et animali mediante libero, Smif8 (Smif8 PGD 2×, suppletum cum 5 mM glutamine, et 8 mM pyruvate), speciatim ad colendam suspensionis cellularum MDCK [95].
Augmentum et contagione experimenta ad exemplar sanationis fiebant adhibitis 500 mL lagunculis quassantibus (#4113-0500, Nalgene™, Thermo Scientific, Waltham, MA; USA) cum cultura initiali voluminis 200 mL ad 150 rpm. Duo culturae fiebant, una infectio-infectio (Cultus 1), ubi cellulae circiter 200 h creverunt, et una infectio (Cultus 2), ubi cellulae IAV fere 49 h post inoculationem infectae sunt. Ad infectio synchrona cellulae incolarum, a moi = 10 adhibita est. Ob retractationem cellularum relative humilis tempore infectionis (2.1 × 106 cellae/mL) et moi altae adhibitae, neque medium tortor neque trypsin additamentum (ad activum virus) necessarium erat. In utraque cultura, aqua Milli-Q sterili addita est antequam sampling ad restitutionem aquae evaporationis (1-2 mL/day) ex quo experimentum in incubatore non hydrato fiebat.
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https:/ /www.mdpi.com/2218-1989/12/3/239/htmhttps://www.mdpi.com/2218-1989/12/3/239/htm
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.