English
The ___@ structura culturae cellae consumables multi-strata est. Communes notationes includunt 1 stratum, 2 laminis, 5 laminis, 10 laminis, 40 stratis, etc. Superior numerus laminis, maior area culturae. Haec consumabilis maxime adhibetur ad culturam cellularum adherentium, ubi hae condiciones necessariae sunt ad incrementum cellularum: 1. cellula officina
Sterile environmentNontoxicitas et sterilitas condiciones magnae sunt ad colendas cellulas in officinis cellis. In vivo, systematis detoxificationis et systematis immunis incursui microorganismi vel aliis substantiis noxiis resistere potest, at in processu culturae vitro, cellae tutela immunis corporis carent ac facultatem defendendi contra microorganismos amittunt et noxias substantias detrahunt. . Curet ut cellulae in vitro ambitu augeri et effingere possint, opus est ut in opere sterili area, valetudinis hygiene personalis, reagentes steriles et media, et aseptica tractatio.
2. Temperatura recta
Plerumque, temperies mammalium et aviarum in vitro colendis apta est 37-38°C. Inepta temperies ambientium cellularum incrementum afficiet. Tolerantia cellularum ad caliditatem frigiditatem fortius est quam caliditas calidi. In temperatura humilis, metabolicae actionis et capacitatis mitoticae cellularum reducuntur. Si siccus non est inferior quam 0 °C, quamvis metabolismus cellula afficitur, detrimentum non est; ad 25 ad 35°C, cellulae crescunt lento gradu; si vero ad 40 °C per aliquot horas posita, non solum iniquum est pro cellae salute et incremento. atque etiam ad mortem. Apta pressio osmotica
solutiones hypertonica vel hypotonica causare possunt cellulas complicare, tumescere, rumpi. Pressio ergo osmotica una est ex condicionibus magni momenti ad cellulas colendas in vitro. Plurimae cellulae in vitro excultae quamdam tolerantiam habent ad pressionem osmoticam. In applicationibus, pressio osmotica 260-320 mmol/L pluribus cellulis applicari potest.
4. Gas environment et pH
The in vitro cultura cellularum specimen gasi environment requirit. Oxygeni et dioxidis carbonis condiciones necessariae sunt ad cellam superstes. Oxygen cyclum cellarum acidum tricarboxylicum participat, energiam pro salute cella, metabolismi et synthesi praebens; dioxydum carbonii non solum est metabolitum cellularum, elementum essentiale ad incrementum cellularum, sed etiam ad pH culturae medium conservandum. Spatium pH maxime idoneum cellularum est saepe 7.2 ad 7.4. In aperto cultura, 5% ratio gas dioxidis carbonii convenit. @___@ Illae quattuor condiciones maiores sunt ad cellulas excolendas in officinis cellulis. Hae condiciones environmentales sunt. Praeterea incrementum cellularum etiam addere debet Serum, medium culturae et alia nutrimenta quae ad incrementum cellularum requiruntur, quod etiam factor praecipuus est ut incrementum cellularum normalium curet.
4. Gas environment and pH
The in vitro culture of cells requires an ideal gas environment. Oxygen and carbon dioxide are necessary conditions for cell survival. Oxygen participates in the tricarboxylic acid cycle of cells, providing energy for cell survival, metabolism and synthesis; carbon dioxide is not only a metabolite of cells, an essential component for cell growth, but also related to maintaining the pH of the culture medium. The suitable pH range for most cells is often 7.2 to 7.4. In the open culture, 5% carbon dioxide gas ratio is appropriate.
The above are the four major conditions for culturing cells in cell factories. These conditions are environmental. In addition, cell growth also needs to add serum, culture medium and other nutrients required for cell growth, which is also a key factor to ensure normal cell growth.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.