English
Cytotoxicitas est eventus mere cell-occisiendi causatus a cellis vel chemicis quae apoptosin vel necrosi non nititur ut mechanismum cella mortis. Hoc experimento iudicari potest utrum rudimenta materiae officina cell continent substantias quae ad cellam incrementum non conducunt.
Cytotoxicitas effectus est substantiarum chemicarum (medicinae) in structuram fundamentalem ac /vel processum physiologicum cellularum, ut membranam cellularum vel structuram cytoskeleton, processus metabolismi cellularum, synthesis, degradatio vel emissio partium cellularum vel productorum, ion ordinatio et divisio cellae et alii processus, ducens ad perturbationes cellae superstites, multiplicationes et/vel munus, unde in adversis profectae. Plerumque probans utrum officinas cellae cytotoxica sit his modis fieri posse:
1. MTT et XTT methodi: activum enzymorum intra mitochondriacum, salia tetrazolii specifici converti possunt, et deinde a lectore microplato deprehenduntur;
2. methodus LDH: cytotoxicitas deprehenditur detegendo enzyme activitatem LDH in cultura cellulae supernatantis;
3. Aliae enzymaticae methodi: ut actiones phosphatasi alcalini et acidi phosphatasi in supernatantis detectis;
4. Cell Proliferation Analysis Kit: Cellae normales validam metabolismum habent, et dehydrogenas succinatas in mitochondria sua salia tetrazolium reducere possunt (ut MTT, XTT, WST-1, etc.) ad substantias cristallinas purpureas circa cellulas depositas ac deinde OD legunt. valorem per microplatam lectorem ad deprehendendam cellulam proliferationis statum.
5. Deprehensio cellae viability per methodum fluorescein lucidum: Adenylate kinasus (AK) est in cytoplasmo omnium cellularum eukaryoticorum et prokaryoticorum, et AK movere potest ADP ad generandum ATP. Cum cellulae laeduntur, membrana cellulae laeditur et AK in culturam supernatantem liberatur. Ornamentum luciferasis et luciferinis utitur ad lucem emittendi sub actione ATP, et quantita- tive deprehendi potest per instrumentum chemiluminiscentiae.
Testis an officinas cellae cytotoxicitas pluribus modis exerceri possit, et haec probatio etiam una est. exercitatione probatio items ut qualitatem cellae culturae consumables curet.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.