English
MTT methodus, quae etiam MTT methodi colorimetricaeest instrumentum quoddam biologicum. Dividitur in fundum planum et fundum rotundum secundum formam. Puteorum numerus comprehendit 6 puteos, 12 puteos, 24 puteos, 48 s. interdum retrahitur, 96 bene bracteae vulgo ad detectionem MTT adhibentur. , modus est ad cellulam superstes et incrementum detegendas. Principium detectionis est quod dehydrogenas succinatas in mitochondriaco vivarum cellularum exogenarum MTT reducere potest ad formazanum crystallum caeruleo-purpureum insolubile (Formazan) et in cellulis deponere, cum cellulae mortuae nullum tale munus habeant. Dimethyl sulfoxidum (DMSO) formazan in cellis dissolvere potest, eiusque lucis effusio aestimatur in 570nm cum enzyme-coniuncto immunoassay, quod indirecte numerum cellularum viventium reflectere potest. Intra certum numerum cellularum numerorum, quantitas formationis crystalli MTT numero cellularum proportionalis est. Gradus detectio sunt haec:
1. Cellulae in @___@Culture @___@. Post cellam densitatem convenit, cuilibet bene 50ul MTT adde et ad culturam pergo per 4 horas;96-bractea culturae bene cellae
2.
Abstrahe culturae medium continens MTT et 150ul addere ex DMSO. Vibrant horizontaliter per 10 minutas ut crystalla plene dissolvant. Cellulae suspensae, post centrifugam, medium 3. Metire absorpiam in 550nm in instrumento enzyme-coniuncto immunoassay (coetus coetus rationabilis et coetus blank constitue); sugunt, et postea DMSO ad dissolvendum adde;
4. Cellula superstes rate=absorbantia valoris experimentalis coetus/absorbentiae valor moderandi coetus*100%.
Notandum in operatione quae MTT est carcinogenica. Si fieri potest, tenta quaedam caestus pelluciduli pelliculae deferant. Paratus MTT sterilis esse debet. MTT bacteria valde sensilis est. Nihil refert, si addas ad laminam culturam bene 96-cellularum sine praesidio a luce. Denique, tempus breve est, aut solatium accendendi averte potes.suck away the medium, and then add DMSO to dissolve;
3. Measure the absorbance at 550nm on the enzyme-linked immunoassay instrument (set a reasonable control group and blank group);
4. Cell survival rate=absorbance value of experimental group/absorbance value of control group*100%.
It should be noted during the operation that MTT is carcinogenic. If possible, try to wear the kind of transparent film gloves. The prepared MTT needs to be sterile. MTT is very sensitive to bacteria. It does not matter if you add to the 96-well cell culture plate without protecting it from light. , After all, the time is short, or you can turn off the lighting on the console.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.