English
Collagenase udvindes fra Clostridium histolyticum og hydrolyserer hovedsageligt kollagen i bindevæv. Når vævet, der skal fordøjes, er hårdt og indeholder meget bindevæv eller kollagen, er effekten af trypsin-dissociation af celler dårlig, og collagenase-dissociationsmetoden kan bruges på dette tidspunkt. Kollagenase har kun en fordøjelseseffekt på det intercellulære stof og har ringe effekt på epitelceller. Derfor er den velegnet til fordøjelse og adskillelse af fibrøst væv, epitel og kræftvæv og kan adskille epitelceller og kollagenkomponenter uden skader. Den sædvanlige dosis er 200U/ml (ca. 1mg/ml) eller 0,03%~0,3%. Kollagenase er opdelt i type Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ og speciel kollagenase til hepatocytter. Kollagenasetypen bør vælges i henhold til celletypen i cellekulturkolben.bruges mere i dyrkningen af adhærente celler. Når celletætheden når et vist niveau, skal vi udføre cellepassageoperationen. Da de klæbende celler er knyttet til bunden af flasken for at vokse, er der behov for fordøjelsesenzymer på dette tidspunkt, og kollagenase er en almindeligt anvendt. navnet på kollagenase er collagenase (Collagenase), som specifikt kan hydrolysere den tredimensionelle spiralformede struktur af naturligt kollagen under fysiologiske pH- og temperaturforhold uden at beskadige andre proteiner og væv. Den kemiske natur af kollagenase er et protein, så det er meget følsomt over for temperatur, pH og forskellige faktorer, der fører til proteindenaturering, og det er meget modtageligt for ydre forhold til at ændre sin egen konformation og egenskaber.
FuDau T75 cellekulturflasker
Ovenstående er den relevante information om nøglekollagenaser og relevante celletyper. Derudover bruges trypsin også til at fordøje celler i cellekulturkolber, som også er et enzym med en relativt høj forbrugshastighed.
Collagenase is extracted from Clostridium histolyticum and mainly hydrolyzes collagen in connective tissue. When the tissue to be digested is hard and contains a lot of connective tissue or collagen, the effect of trypsin dissociation of cells is poor, and collagenase dissociation method can be used at this time. Collagenase only has a digestive effect on the intercellular substance and has little effect on epithelial cells. Therefore, it is suitable for digestion and separation of fibrous tissue, epithelium and cancer tissue, and can separate epithelial cells and collagen components without damage. The usual dose is 200U/ml (about 1mg/mL) or 0.03%~0.3%. Collagenase is divided into type Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ and special collagenase for hepatocytes. The type of collagenase should be selected according to the cell type in the cell culture flask.
FuDau T75 Cell Culture Flasks
The above is the relevant information of key collagenases and applicable cell types. In addition, trypsin is also used to digest cells in cell culture flasks, which is also an enzyme with a relatively high usage rate.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.