Sut i ddelio ag "effaith ymyl" platiau 96-ffynnon?

Wed May 18 14:18:45 CST 2022

Mae "effaith ymyl" yn sefyllfa a wynebir yn aml yn ystod y defnydd o 2. Defnyddiwch orchudd gwrth-anweddol: Mae rhai pobl wedi datblygu gorchudd tyllog gwrth-anweddol ar gyfer yr effaith ymyl a achosir gan effaith anweddoli'r yn enwedig mewn imiwn-ymatebion sy'n gysylltiedig ag ensymau (arbrofion ELISA). Bydd y sefyllfa hon yn achosi i gyfradd twf y celloedd yn y tyllau ymyl arafu, sy'n effeithio'n uniongyrchol ar ganlyniadau'r prawf. 1 . Cyn deori: Defnyddiwch faddon dŵr neu wrth ychwanegu'r hydoddiant adwaith at blât 96-ffynnon, cynheswch y plât a'r hydoddiant i'r tymheredd deori (fel 37 ° C), a all ddileu'r "effaith ymyl" yn hawdd a gwella'r ailadroddadwyedd y mesuriad. Yn ogystal, mae rhai astudiaethau wedi canfod y gall gosod y celloedd palmantog ar dymheredd ystafell am awr a'u dychwelyd i'r deorydd 37 ° C i barhau i feithrin ar ôl i'r celloedd setlo a glynu wrth y wal hefyd wella'r effaith ymyl yn sylweddol.

. Gall y gorchudd plât tyllog ffurfio haen anwedd dŵr dirlawn ar y plât tyllog i ddod yn darian stêm, ac ynysu'r amgylchedd y tu mewn i'r plât tyllog o amgylchedd y labordy, er mwyn cynnal y plât tyllog yn well mewn cyflwr cymharol sefydlog.@__ plât @mandyllog

3. Rhoi'r gorau i'r tyllau ymylol: Pan na ellir datrys y dulliau uchod, er mwyn cael canlyniadau profion mwy dibynadwy, y dull mwyaf syml a syml yw peidio â defnyddio'r tyllau ymylol hyn.

Mae'r uchod yn atebion i atal effeithiau ymyl yn 96-well. Gall meistroli'r dulliau prosesu hyn leihau effaith effeithiau ymyl ar blatiau canlyniadau prawf.

3. Abandon the peripheral holes: When the above methods cannot be solved, in order to obtain more reliable test results, the most straightforward and simple method is to not use these peripheral holes.

The above are solutions to prevent edge effects in 96-well plates. Mastering these processing methods can reduce the impact of edge effects on the test results.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.